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The dissolution of double holliday junctions

WebAlternatively, double Holliday junctions can be processed by a reaction known as “double Holliday junction dissolution.” This reaction requires the cooperative action of a so-called “dissolvasome” comprising a Holliday junction branch migration enzyme (Sgs1/BLM RecQ helicase) and a type IA topoisomerase (Top3/TopoIIIα) in complex with ... WebJun 30, 2005 · Double Holliday junction dissolution is specific for BLM. Despite the similar biochemical properties of many RecQ helicases in vitro, and their ability to act upon a wide variety of DNA structures, an elevated frequency of SCEs is thought to be a feature unique to BLM-deficient cells among human RecQ helicase mutants.

Dissolution of Double Holliday Junctions by the Concerted Action …

WebJul 9, 2014 · The dissolvasome complex collapses and unlinks a double Holliday junction DNA double-strand breaks can be repaired by homologous recombination, whose key intermediate is the four-way Holliday junction. During meiosis, Holliday junctions are cut by endonucleases to generate crossovers that are key to the proper segregation of … WebJul 9, 2014 · The dissolvasome complex collapses and unlinks a double Holliday junction DNA double-strand breaks can be repaired by homologous recombination, whose key … p buckley moss ohio star https://adwtrucks.com

Dissolution of double Holliday junctions by the concerted …

Webactivities include ATP-dependent Holliday junction migration, 3'-5' strand displacement, and ATP-independent single-strand annealing (9, 10). Most significantly, BLM protein in conjunction with topoisomerase IIIa (Top3a) can resolve double Holliday junctions (dHJs) via dissolution or convergent migration (11-14). WebJul 1, 2014 · Coronavirus: Find the latest articles and preprints ... WebDescription: Homo sapiens RAD51 associated protein 1 (RAD51AP1), transcript variant 1, mRNA. Transcript (Including UTRs) Position: hg19 chr12:4,647,950-4,669,213 Size ... scriptures says as a man thinks in his heart

The Dissolution of Double Holliday Junctions

Category:Rmi1 stimulates decatenation of double Holliday junctions during ...

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The dissolution of double holliday junctions

Holliday junction resolution: Regulation in space and time

WebOct 10, 2010 · Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and... WebJun 30, 2005 · Double Holliday junction dissolution is specific for BLM Despite the similar biochemical properties of many RecQ helicases in vitro, and their ability to act upon a …

The dissolution of double holliday junctions

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WebMay 3, 2024 · HJs (double Holliday junctions [dHJs]) converge in an adenosine triphosphate (ATP)- hydrolysis – dep endent r eacti on to form a h emicat enan e that is su bseque ntly de caten ated by ... WebOct 10, 2010 · Sgs1 and Top3, stimulated by replication protein A (RPA), dissolve double Holliday junctions to yield non-crossover products. (a) A schematic representation of the double Holliday junction substrate (DHJS) showing dissolution by convergent branch migration or resolution by nucleolytic cleavage.Dissolution of the dHJ leads exclusively to …

WebJan 1, 2009 · The dissolution of double Holliday junction requires the concerted action of BLM and topoisomerase IIIα (compare lane 17 with lanes 5 and 6). Human RMI1 … WebDouble Holliday junctions (dHJS) are important intermediates of homologous recombination. The separate junctions can each be cleaved by DNA structure-selective …

WebThe Holliday junction is a key intermediate in homologous recombination, a biological process that increases genetic diversity by shifting genes between two chromosomes, as … WebThe resolution of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintenance and proper chromosome segregation. ... DNA joint molecules arise during the recombinational repair of DNA double-strand breaks (DSBs) and form a physical connection between sister chromatids or homologous chromosomes ...

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scriptures seldom preachedWebA double dissolution is a procedure permitted under the Australian Constitution to resolve deadlocks in the bicameral Parliament of Australia between the House of Representatives … p buckley moss pinsWebJan 2, 2014 · Although Blm and Top3α are known to form a minimal dissolvasome that can uniquely undo a double Holliday junction structure, the details of the mechanism remain unknown. It was originally suggested that Blm acts first to create a hemicatenane structure from branch migration of the junctions, followed by Top3α performing strand passage to … p. buckley moss pinsWebParticipates in DNA replication and repair. Involved in 5"-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5"-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. scriptures sex before marriageWebIn mitotic cells, double HJs are primarily dissolved by the BLM helicase-TopoisomeraseIIIα-RMI1-RMI2 (BTR) complex, whereas single HJs (and double HJs that have escaped the … scriptures shopWebIn this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro. Based on techniques Marjana Tomic-Canic 1996, Springer Protocols scriptures seeking god for directionWebDescription: Homo sapiens WRN RecQ like helicase (WRN), mRNA. (from RefSeq NM_000553) RefSeq Summary (NM_000553): This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription … scriptures set to music