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Web2 μl Strand-Switching Primer (SSP, at 10 µM) Mix gently by flicking the tube, and spin down. Add the strand-switching buffer to the snap-cooled, annealed mRNA, mix by flicking the … Webhexamer primer in the synthesis of the first cDNA strand and DNA–RNA template-switching primers during the synthesis of the second cDNA strand (Cloonan et al., 2008). Another … pediatric oropharyngeal dysphagia
PCR-cDNA Barcoding (SQK-PCB109)
WebTaking full-length poly-A+ RNA, complementary strand synthesis and strand switching are performed using kit-supplied oligonucleotides. The kit contains 12 primer pairs which are … Web1 Mar 1999 · Different template-switching oligos and PCR primers were used in each experiment: lanes A, ‘TS-short’ and ‘TS-PCR’ primer; lanes B, ‘TS-long’ and ‘heel-specific’ … Web4.3 2 μl Strand-Switching Primer (SSP, at 10 μM) 5 Mix gently by flicking the tube, and spin down. 6 Add the strand-switching buffer to the snap-cooled, annealed mRNA, mix by … meaning of tender eyed