Lysosensor yellow/blue dextran
Web3,000 MW dextran conjugates may be preferred in some appli-cations because they penetrate peripheral neuronal processes better and diffuse faster than higher-MW dextrans.29,30 Conjugate Ex (nm)* Em (nm)* LysoSensor™ Yellow/Blue† 335, 381 452, 521 Indo‡ 356, 343 466, 408 Fura‡ 364, 340 503, 495 Fluo-4 494 516 Oregon ®Green … WebSummary of Our LysoTracker and LysoSensor Probes—Table 12.3 Learn more in The Molecular Probes® Handbook Probes for Lysosomes, Peroxisomes and Yeast Vacuoles—Section 12.3 And visit these featured pages Organelle Stain Selection Guides Table 12.3 Summary of our LysoTracker and LysoSensor probes. For Research Use …
Lysosensor yellow/blue dextran
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WebLysosensor Yellow Blue Dextran, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Web17 aug. 2024 · To test this, lysosomes were loaded with dextran-conjugated lysosensor yellow/blue DND-160 (lysosensor), a ratiometric dye that allows absolute quantification of lysosomal pH (Supplementary...
WebOregon Green and LysoSensor Yellow/Blue Dextrans Although the fluorescein, BCECF and SNARF dextrans are intended for pH measurements between pH ~6 and 8, these … WebMethods: A ratiometric method by confocal laser scanning microscopy with Lysosensor yellow/blue dextran was adapted from those previously reported, and micropH distribution kinetics inside microspheres was examined during incubation under …
Web12 iul. 2008 · Confocal Imaging of Lysosensor Yellow/Blue® and Image Processing. The critical importance of the use of dye conjugated with high molecular weight (10 kDa) dextran is to make the dye a long-term tracer and water-soluble macromolecule, which can prevent dye leaching out from polymer, and localize the dye in the aqueous pores similar to the … WebLysoSensor探针是细胞内的pH荧光指示剂，可通过质子化作用聚集在酸性细胞器内。 这个质子化也减弱染料的荧光淬灭性，从而增加荧光强度。 因此，与LysoTracker系列探针相比，LysoSensor系列探针随着细胞器的酸化程度增强，其荧光强度呈极大的pH依赖型 (向酸)增强。 产品选择指南 注：探针标记细胞后，只能用荧光显微镜观察，而不能用流式或荧光 …
Web27 apr. 2013 · LysoSensor™ Yellow/Blue dextran, a pH-sensitive indicator with fluorescence excited at two channels, was encapsulated into dextran nanoparticles to …
Web9 ian. 2024 · The researchers then used the pH-sensitive LysoSensor Yellow/Blue dextran to measure lysosomal acidification, which revealed a significantly higher lysosomal pH in the AD neurons than in the... offline adaptive radiotherapyWebIn my experience from working on this, the Lysosensor Dyes are really finicky. I don't have experience with the Lysosensor Yellow/blue dextran but I have worked with Lysosensor green. The lysotracker dyes work extremely well … offline address book connecting to exchangeWeb1 dec. 2024 · For Lysosensor yellow/blue dextran, the correlation of fluorescence intensity ratio to pH was calibrated in the presence of exenatide. A range of concentrations of exenatide solution (i.e., 25 mg/mL – 200 mg/mL) were prepared by dissolving exenatide in the universal buffers of pH 2.8 to 5.8. Acetic acid and sodium hydroxide were used to ... offline action games for laptopWebLysoSensor Yellow/Blue dextran can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is taken up by the cells through endocytosis. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue ... offline address book downloadWebFor comparison, we also labelled HeLa cells with 500 μg ml−1 of the commercial LysoSensor™ Yellow/Blue dextran (LSYB, ThermoFisher) for 18–24 h at 37 °C, 5% CO2 in complete FluoroBrite™ DMEM (see above). myers 7 coWeb15 ian. 2009 · LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior … myers 76 station incWebLysoSensor Yellow/Blue dextran is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is... Shop offline address book m365