Immortalized human podocyte cell line
Witryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher …
Immortalized human podocyte cell line
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WitrynaTo investigate changes on the cellular level, we used an immortalized human podocyte cell line and generated a stable knockdown of Sphk2 (Sphk2-kd) by a lentiviral transduction method. WitrynaPlating of glomeruli onto a collagen surface is followed by an outgrowth of cobblestone-like cells that, after replating, differentiate into arborized, mature podocytes. Currently, the majority of research studies use immortalized podocytic cell lines most often derived from transgenic mice bearing a conditional immortalizing gene.
Witryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of … WitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein …
WitrynaImmortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T … Witryna18 kwi 2024 · The immortalized human podocyte cell line were purchased from Bristol University and cultured under their published protocol. Podocyte mRNA profiles of these cells were examined. Contributor(s) Yoshimura Y, Nishinakamura R: Citation(s) 30635375: Submission date: Jun 30, 2024: Last update date: Apr 18, 2024:
WitrynaIn contrast, no significant change was observed in mesangial cells after 12 h, and even after 48 h the wounds were not completely closed. Until now, conditionally …
WitrynaExperimental approach: Expression of TRPV1-4 channels was investigated at protein (immunocytochemistry, Western blot) and mRNA (Q-PCR) level in a conditionally immortalized human podocyte cell line. Channel function was assessed by measuring intracellular Ca 2+ concentration using Flou-4 Ca 2+ -indicator dye and patch clamp … オイルフィルター レンチ 使い方WitrynaOther investigators have immortalized mouse and human neural stem cells using retroviral transduction of the v-myc oncogene (Snyder, 1995; ... 2007) and his team … オイルフィルター レンチ 滑るWitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, … paolo vecchiettiWitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation. オイルフィルター レンチ 適合表Witryna1 mar 2010 · Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term urinary cell … オイルフィルター 先WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more オイルフィルター 互換 表WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … paolo veltri