Web12 apr. 2024 · Screening of in vitro antidiabetic assay in HepG2 cells A glucose uptake assay was conducted in insulin resistance HepG2 (IR-HepG2) cells to establish the antidiabetic activity of the Lc-EAF. IR-HepG2 cells were first generated according to the protocol reported by Saravanakumar et al. ( 2024 ). WebThe insulin‑induced expression of GLUT2 and p‑GSK‑3 were eliminated by 3DG (80 and 300 ng/ml), in addition to inhibiting the phosphorylation of downstream effectors of the …
Suitability of hepatocyte cell lines HepG2, AML12 and THLE-2 for ...
Web14 apr. 2024 · We show SESN2 competes with insulin like growth factor 2 mRNA ... including metabolic syndrome diseases such as insulin resistance and lipid … Web2 dagen geleden · Three insulin resistance models in vitro were established by using TNF-α, insulin and palmitic acid (PA) in HepG2 cells, respectively. We compared the effects … hole to screw chart
Carpachromene Ameliorates Insulin Resistance in HepG2 Cells via ...
WebInsulin-stimulated protein kinase B (Akt/PKB) phosphorylation was measured in high glucose-induced, insulin-resistant HepG2 cells. Similar to 30 μmol/l rosiglitazone, … Web2 dagen geleden · HepG2 cells were treated with FFA to establish the insulin resistance model. Then, the cells were treated with SC79 (10 μmol/L), CA (10, 1, and 0.1 μmol/L), CA4G (10, 1, and 0.1 μmol/L), FA (10, 1, and 0.1 μmol/L), or FA4G (10, 1, and 0.1 μmol/L) respectively for 2 h, according to our preliminary experiment (data not shown). WebThe HepG2 cells were washed with phosphate buffered saline (PBS) and fixed with ORO Fixative for 1 h. Cells were rinsed with ddH2O, dipped in 60% isopropanol for 5 min, … huff 2 bluff marathon