WebDec 29, 2024 · Go to: Abstract Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing of haploid gametes enabling separation of the whole-genome sequencing reads into haplotype-specific reads sets. WebWant to thank TFD for its existence? Tell a friend about us, add a link to this page, or visit the webmaster's page for free fun content. Link to this page:
Genome Sequencing of - Oxford Academic
WebJan 10, 2024 · GenomeScope2 44 and findGSE 45 with different k-mer sizes (k = 21, 23, 25, and 27) as well as MGSE software 46 were employed to predict genome size. The genome size was estimated with the formula: Genome_Size = K-mer coverage/Mean k-mer depth 47. Genome assembly and SSR marker development. WebOct 12, 2024 · Siluriformes (catfish) is an order of major aquaculture species worldwide, especially in China, the United States, and Vietnam ( De Silva and Phuong, 2011; Zhong et al., 2016; Kumar et al., 2024 ). Red-tail catfish ( Hemibagrus wyckioides ), belonging to the family Bagridae, initially possess a white caudal fin that becomes bright red when it ... good life background
Chromosome-Level Genome Assembly of the Asian Red-Tail …
WebJul 20, 2024 · Genome assembly assessment and comparison. (A) k-mer frequency histogram output from findGSE using k = 21.The gray line represents the observed k-mer frequency, the teal line represents the fit for the heterozygous k-mer peak, the blue line represents the fitted model without k-mer correction, and the red line represents the fitted … WebMar 3, 2024 · The k-mer histogram was provided to findGSE (v.1.0) 39 to estimate the haploid/tetraploid genome size of ‘Otava’ under the heterozygous mode (with ‘exp_hom = 200’; Extended Data Fig. 1). WebSep 1, 2024 · K-mer frequency distributions of 21-, 25-, 31-, 41-, 65-mers were generated, then we used findGSE (Sun et al. 2024) to estimate genome size and repeat content. Repeat content analysis To identify repeats in the genome assembly, a custom species-specific repeat library was created, using the RepeatModeler package 1.0.8 ( Smit 2010 ) … good life bakery