WebAug 24, 2024 · The COP1–HY5 regulatory module is crucial for regulating cold acclimation in plants ( Catala et al., 2011 ). Interactions among CRY2, COP1, and SPA inhibit the E3 ubiquitin ligase activity of CULLIN4 … WebCOP1 targets numerous substrates for ubiquitination and pro-motes their proteolysis via the 26S proteasome system (Hoecker, ... CRY1,2 Photoreceptors HY5 HYH HFR1 Photomorphogenesis BBXs PIFs phyA phyB phyA phyB DET1 COP1 COP1 SPAx SPAy DDB1 COP10 etc. Physiological output CUL4 CRY1,2 Light signals
The photomorphogenic repressors COP1 and DET1: 20 years later
We wish to understand different mechanisms governing the activities of COP1 and LRBs with respect to CRY2 ubiquitination. Most presently known light-dependent CRY2-interacting proteins interact with the photon-sensing PHR domain of CRY23. However, COP1 is one of the few exceptions. COP1 binds to the … See more We have previously shown that the blue light-dependent ubiquitination and proteolysis of CRY2 is diminished but not completely abolished in the cop1 null mutant22, suggesting the existence of another E3 ubiquitin … See more To test whether the Cul3LRBs and Cul4COP1/SPAs E3 ubiquitin ligases catalyze blue light-dependent CRY2 ubiquitination, we performed three independent experiments. In the first two experiments, we … See more We next tested how LRBs regulate cryptochrome activity by examining the blue light-dependent degradation of CRY2 in the lrb123 mutants. Results shown in Fig. 2 demonstrate … See more Given that a substrate receptor of E3 ubiquitin ligases must physically interact with the substrate, we examined the CRY2/LRB interaction (Fig. 3), using the co-immunoprecipitation (co-IP) assays of proteins co … See more WebMar 29, 2024 · The circadian clock and the hypoxia-signaling pathway are regulated by an integrated interplay of positive and negative feedback limbs that incorporate energy … fa komposztáló láda
COP1 and BBXs‐HY5‐mediated light signal transduction in …
WebOct 1, 2012 · In blue light, cry1 interacts with SPA1 and sequesters it from COP1, thereby probably disrupting the COP1–SPA1 complex that is important for E3 function. COP1 also interacts with cry1 in a light-independent manner (not shown here). Visible light further promotes the nuclear export of COP1 for long-term suppression of COP1. WebJan 4, 2024 · Plants encode three different CRYs (CRY1–3); ... R. H. & Cashmore, A. R. The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. Plant Cell 13, 2573–2587 (2001). WebNational Center for Biotechnology Information hisun 500 atv manual