Clontech y1h
WebAdron Ung. So say that for an experiment which requires a [DNA] / [Protein] = 1.2 / 1 ratio, I have calculated that I need 96.2296 uM of duplex DNA. This double stranded DNA is formed by annealing ... WebI used the Matchmaker Gold yeast one-hybrid system from Clontech. ... The p53 fragment was from pGBKT7-p53 used for Y2H. I don’t know whether this fragment is suitable for …
Clontech y1h
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Web丁香通为您找到319条产检dna序列信息，包括产检dna序列报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购产检dna序列,上丁香通。 WebAug 1, 2024 · The coding sequence (CDS) of ThWRKY4 was cloned into vector PGADT7-Rec2 as the bait (Clontech, CA, USA) (see Supporting information Table S1 for primers). Y1H screening was performed to determine the cis-acing elements bound by ThWRKY4 (GenBank number: JX416193). The positive clones were further subjected to high …
WebJun 6, 2014 · Identification and cloning of tomato TCP genes. By mining the tomato Unigene and BAC sequence databases from the Sol Genomics Network with homology searches and subsequent sequence extension by RACE, we initially identified and cloned 24 different tomato genes encoding putative TCP transcription factors (Table 1; SlTCP1 … WebJun 28, 2024 · Y1H assays were performed using the Matchmaker Gold Y1H System (Clontech, CA, USA) according to the manufacturer’s protocol. Total RNAs were extracted using Trizol (Invitrogen) from the mixed leaves, stems, flowers, fruits, and storage roots of cassava. Synthesis of the cassava double-stranded cDNA library was constructed …
WebApr 5, 2024 · Y1H assays. The full-length CDS of LkARF7 and LkARF19 were cloned into the prey vector pGADT7 individually, ... (Clontech, USA). The cDNA library was screened on SD/-Trp-Leu, SD/-Trp-Leu-His, and SD/-Trp-Leu-His-Ade/X-α-Gal medium to identify LkARF19-interacting proteins. The positive clones interacting with LkARF19 were … WebClosed Date 2005. Founded Date 1974. Founders Roger Tsien. Operating Status Closed. Last Funding Type Private Equity. Company Type For Profit. Contact Email …
WebFeb 22, 2006 · Y1H reporter strains were prepared by cloning three to four tandem repeats of: 1) the cis-elements specific for two classes of homeodomain transcription factors (HDZipI-II) [23, 24]; 2) the Drought Responsive Element (DRE) from the promoter of the rd29A gene from Arabidopsis thaliana, specific for the DREB/CBF subfamily of single …
WebA Y1H assay requires that a prey construct is introduced into a bait yeast strain that harbors the reporter construct(s). This is commonly achieved by transforming the expression construct into a haploid bait yeast strain. However, this can also be achieved using the ability of haploid yeast strains to mate and generate diploid yeast. get well wishes for coworker family memberWebOct 1, 2004 · We tested whether Gateway AttB recombination sites influence the readout of the Y1H system, for example, by binding an endogenous yeast TF. We compared our system to the commercially available Clontech Y1H system (BD Biosciences). As a positive control, we used the interaction between the TF p53 and its consensus DNA-binding site. get well wishes for an employeeWebIn the Y1H assay, potential target genes of a TF may be screened out by cis-element analysis. Similarly, the bind-ing site can also be identified with known TFs cDNA. Wang … get well wishes for a friendWebAbstract. One-hybrid screening in yeast is a powerful method to rapidly identify heterologous transcription factors that can interact with a specific regulatory DNA sequence of interest (the bait sequence). In this technique, the interaction between two proteins (bait and prey) is detected via in vivo reconstitution of a transcriptional ... christopher redlich jrWebJun 1, 2024 · The yeast one-hybrid (Y1H) is a powerful and widely used gene-centered system to identify DNA–protein interactions. However, a powerful and simple TF … christopher redmann attorney bismarck ndWebIn the Y1H assay, potential target genes of a TF may be screened out by cis-element analysis. Similarly, the bind-ing site can also be identified with known TFs cDNA. Wang et al. [14] has modified an inverse Y1H assay to screen the candidate target genes of WRKY33 when studying the role of Arabidopsis WRKY33 in drought. In get well wishes for a friend imagesWeb菌落PCR产品，验证整合到Y1H酵母基因组的bait DNA序列 . 品牌：Clontech 技术资料：0 篇 【商品总览】Clontech【630495】酵母双杂培养基套装（含 ... get well wishes for coworker after surgery