Cholesterol efflux assay kit cell-based
WebAtherosclerosis (AS) is a common cardiovascular disease and remains the leading cause of death in the world. It is generally believed that the deposition of foam cells in the arterial wall is the main cause of AS. Moreover, promoting cholesterol efflux and enhancing the ability of reverse cholesterol transport (RCT) can effectively inhibit the formation of foam cells, … WebJun 25, 2024 · Cholesterol efflux was determined using a cell-based Cholesterol Efflux Assay kit (Abcam, Cambridge, UK) according to manufacturer’s instructions. Activated cells were labeled with fluorescent cholesterol for 16 h and washed with the RPMI-HSA medium. A total of 5 μg protein weight of the treated HDL samples were added to the culture of ...
Cholesterol efflux assay kit cell-based
Did you know?
WebCholesterol Efflux Assay Kit (Cell-based) (ab196985) is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive and reproducible method for measuring cholesterol efflux. This product can be used to: ... WebFoam cell formation as a result of imbalance of modified cholesterol influx and efflux by macrophages is a key to the occurrence and development of atherosclerosis. Oxidative stress is thought to be involved in the pathogenesis of atherosclerosis. SS-31 is a member of the Szeto-Schiller (SS) peptides shown to specifically target the inner mitochondrial …
WebCholesterol Efflux Fluorometric Assay Kit (cell-based) - 100 assays - K582-100 from BioVision. Tel: +44 (0)1223 316 855; register; log in; view basket: 0 items; ... BioVision’s … Webcommercial kits cholesterol efflux assay kit Abcam, ab196985 9.2 cholesterol efflux assay kit Sigma-Aldrich, MAK192 9.23 cholesterol efflux fluorometric assay kit (cell …
WebCholesterol Efflux Fluorometric Assay Kit (Cell-Based) 1/14 (Catalog # MBS841611; 100 assays; Store at -20°C) I. Introduction: ... Thaw before adding to the cells. VIII. Cholesterol Efflux Assay Protocol: The procedure described below is for macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from WebThe cell culture medium obtained from the cholesterol efflux assay using [d 7]C was centrifuged, and the remaining cells were removed. Cell lysates were prepared by adding 1% sodium cholate solution to cells cultured without extracellular acceptors, followed by incubation for 1 h at room temperature.
WebJan 15, 2024 · Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux. storage instruction : Store the kit at -20 C, protected from light. note : Result Data. type clonality : Kit. species reactivity cross reactivity : Mammals. application key : Func. size : 1 Kit.
WebCholesterol Efflux Fluorometric Assay Kit (Cell-Based) rev 3/19 (Catalog # K582-100; 100 assays; Store at -20°C) I. Introduction: Cholesterol efflux from the peripheral tissues … elizabeth ann tennisWebCell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux. Supplier: Abnova™ KA4542 Catalog No. 89-939-181 elizabeth ann townsendWebPrinciple of the Assay The Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring ... cholesterol efflux from cells using a fluorescently-labeled cholesterol analogue. The kit includes a reagent to remove apolipoprotein B-containing lipoprotein particles (LDL and VLDL) from serum samples ... elizabeth ann wainwright oxford wiscWebCholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides … forcasting the economy investment bankingWebMay 18, 2024 · Macrophage foam cells (i.e., cholesteryl ester-laden macrophages) are abundant in atherosclerotic plaques, and increased macrophage foam cell content is associated with plaque instability (1, 2).Macrophages are generally thought to unload surplus cholesterol via efflux mediated by the ATP-binding cassette transporters A1 … forcast international markets futureWebBioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides … elizabeth ann swainWebBioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides … elizabeth ann walker nlp